KForce Probiotics with BLIS K12 - BreezeCare Oral Health Company

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KForce Breath Guard with BLIS K12


Bad Breath Diagnosis

What is BLIS K12
BLIS K12 is the world's first advanced probiotic for the oral cavity (mouth) and upper respiratory tract. It is a specific strain of Streptococcus salivarius, a common oral bacteria. The strain specific actions of K12 help provide a natural defense against bacterial halitosis. Additionally, K12 can boost our immune system's defenses.

How was BLIS K12 Discovered?
The K12 strain was originally isolated from the mouth of a healthy child who was tracked for several years and did not develop sore throats. Scientists discovered that this particular K12 strain of S. salivarius secretes powerful antimicrobial molecules called BLIS, (Bacteriocin-Like-Inhibitory Substances), with the ability to destroy harmful bacteria such as the bacteria responsible for "strep" sore throat and other upper respiratory infections.

The mouth is an entry point for pathogens as it comes into contact with billions of bacteria. The microorganisms in our mouth are a first line of defense against pathogens and an important part of our immune health. If a healthy balance of oral bacteria is thrown off by stress, exposure to disease or antibiotics, it can quickly lead to problems such as throat infections, ear infections and bad breath. By promoting a healthy balance of bacteria in the mouth and upper respiratory tract, BLIS K12 can help protect the gateway to our body's health, and thus prevent bad breath.

Why is KForce Breath Guard with K12 so Unique?
S. salivarius is one of the most numerous beneficial bacteria found in the mouth of healthy individuals. However, only a small amount of people produce a kind of S.salivarius with BLIS K12 activity. Most probiotic bacteria, including S.salivarius, compete with other microorganisms for space and nutrients in the oral cavity and in this way, act to maintain a healthy balance of oral bacteria. BLIS K12 works beyond competitive inhibition. It secretes two anti-microbial proteins called Salivaricin A and Salivaricin B which destroy undesirable bacteria, making it more effective at fighting off pathogens. When BLIS K12 enters the mouth, it attaches to cells in the oral cavity and colonizes. By colonizing, BLIS K12 crowds out pathogenic bacteria, keeping them from adhering to the host. The final antibacterial punch comes from the production of BLIS molecules, Salivaricins A and B. Many potential pathogens are susceptible to these Salivaricins, including the bacteria that cause sore throats, bad breath and those that cause respiratory tract and ear infections. In addition to its ability to inhibit pathogens, BLIS K12 can communicate with certain cells in the mouth, stimulating them to boost our immune system defenses.

 


KForce Breath Guard with K12 Efficacy This figure illustrates the more potent activity of BLIS K12 in inhibiting the strep throat causing bacteria S. pyogenes as compared to normal S. salivarius.

BreezeCare Clinic Bad Breath Research Paper
To determine whether dosing with bacteriocin-producing Streptococcus salivarius (KForce Breath Guard K12) following an antimicrobial mouthwash effects a change in oral malodour parameters and in the composition of the oral microbiota of subjects with halitosis.

Twenty-three subjects with halitosis undertook a 3-day regimen of chlorhexidine mouth rinsing, followed at intervals by the use of lozenges containing either S. salivarius K12 or placebo. Assessment of the subjects' volatile sulphur compound (VSC) levels 1 week after treatment initiation showed that 85% of the K12-treated group and 30% of the placebo group had substantial (>100 ppb) reductions. The bacterial composition of the saliva was monitored by culture and PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Changes in the PCR-DGGE profiles occurred in most subjects following K12 treatment.

In vitro testing showed that S. salivarius K12 suppressed the growth of black-pigmented bacteria in saliva samples and also in various reference strains of bacteria implicated in halitosis.

Conclusions
Administration of bacteriocin-producing S. salivarius after an oral antimicrobial mouthwash reduces oral VSC levels. The outcome of this preliminary study indicates that the replacement of bacteria implicated in halitosis by colonization with competitive bacteria such as S. salivarius K12 may provide an effective strategy to reduce the severity of halitosis.


KForce Breath Guard K12 Study

During the trial we had two amazing results. One subject dropped their breath score from 1400 to 250 in one week. The second person dropped their breath score from 1150 to 150.

 

Anything under 150 is considered a normal breath reading. These patients were people who had suffered most of their lives with the embarrassment of bad breath.

 

"A preliminary study of the effect of probiotic Streptococcus salivarius K12 on oral malodour parameters" J.P. Burton, C.N. Chilcott, C.J. Moore, G.Speiser, and J.R. Tagg. Journal of Applied Microbiology 100 (2006) 754-764

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